wavelength 595 nm total test time 2 min working reagent stability 18-26°C for 2 weeks dynamic range 50 mg/dL 0.5 g/L
parameter
18-26 °C temperature 50 μL sample volume
storage temp.
2-8°C
General description
Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.
Application
Total protein kit, micro has been used to determine:
total protein in the bacterial homogenate by Bradford method[1]
total protein content by the Bradford microassay from the tissue extracts[2]
soluble protein content by the Bradford method from leaves[4]
Biochem/physiol Actions
Total protein kit, micro can be used to determine the concentration of proteins in solution.
Suitability
Suitable for protein determination for samples of up to 0.5 mg/ml.
Principle
Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.
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