T5701

Anti-TSG101 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Tumor Suspectibility Gene 101
• MDL number: MFCD08705528
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 46 kDa
• species reactivity: rat, mouse, human
• concentration: ~2 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using cell lysates of rat fibroblast Rat-1 and mouse fibroblast NIH3T3 cells; western blot: 2-4 μg/mL using cell lysates of human A431 cells
• UniProt accession no.: Q99816
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TSG101(7251) ; mouse ... Tsg101(22088) ; rat ... Tsg101(292925)

Description

General description

Tumor susceptibility 101 (TSG101) is encoded by the gene mapped to human chromosome 11p15.1–15.2. The encoded protein is a member of catalytically inactive ubiquitin E2 variant (UEV) family. Tsg101 is characterized with an ubiquitin E2 variant (UEV) domain in its N-terminal end involved in HIV-1 budding and the vacuolar protein sorting (VPS) pathway.

Immunogen

syntheitic peptide corresponding to amino acids 254-272 located near the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101.

Application

Anti-TSG101 antibody produced in rabbit has been used in western blot analysis.

Biochem/physiol Actions

Tumor susceptibility 101 (TSG101) is implicated in various cellular function such as, ubiquitination, transcriptional regulation, endosomal trafficking and cell proliferation. Mutation in the gene leads to various human cancers.Tsg101 plays a crucial role in cell growth, cell survival, and normal function of embryonic and adult tissues. TSG101 facilitates infectious human immunodeficiency virus (HIV-1) budding by linking the p6 late domain to vacuolar protein sorting machinery. Tsg101 is a vital constituent of the endosomal sorting complex required for transport (ESCRT) machinery, that helps in synthesis of multi-vesicular bodies, mainly the formation and scission of the intraluminal vesicles, and is thus, crucial for the degradation and recycling of receptors localized on plasma membrane. Tsg101 expression is controlled by an intrinsic autoregulatory mechanism and by ubiquitination catalyzed by various ubiquitin ligases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves