SRP8003

Clusterin (nuclear form) mouse

recombinant, expressed in E. coli, His tagged, >90% (SDS-PAGE)

• Synonym(s): Apolipoprotein J, CLI, Lymphocyte antigen 52 (Ly-52), SGP-2, TRPM-2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• tag: His tagged
• Assay: >90% (SDS-PAGE)
• form: liquid
• mol wt: ~45 kDa by SDS-PAGE
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: ≥0.2 mg/mL
• impurities: <1 EU/μg endotoxin, tested
• color: clear
• UniProt accession no.: Q06890
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Clu(12759)

Description

General description

Clusterin is expressed in all tissues. However, it is strongly expressed in the central nervous system and plasma. Clusterin mainly protects against heat and other stress. It is also referred as chaperone protein and stress-inducible biomarker. Clusterin exists in two isoforms, one is a secretory form and other is a nuclear form. The secreted form of the protein is a 80kDa disulfide-linked heterodimer of α and β subunits (produced by internal cleavage). The alternatively spliced inactive cytosolic form is changed to a truncated mature nuclear isoform via ionizing irradiation.

Biochem/physiol Actions

Clusterin is involved in tissue remodeling, cell death, lipid transport, complement-associated cell lysis, cancer and neurological damage. It is strongly expressed in Alzheimer′s and treatment-resistant cancer. High levels of clusterin are observed in ischemic heart disease and diabetic conditions. It also protects kidney from fibrosis. Nuclear form of clusterin works as a pro-death signal, and suppresses cell growth and survival. The secreted form of clusterin has extracellular chaperone and anti-apoptotic activities.

Physical form

Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl.

Other Notes

Mouse nuclear clusterin (aa 22-448) is fused at the C-terminus to a His-tag.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable