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SRP6145

Sigma-Aldrich

linear tetra-ubiquitin human

Synonym(s):

Ub4

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

form

liquid

mol wt

34.8 kDa (His Tagged)

packaging

pkg of 100 μg

shipped in

dry ice

storage temp.

−70°C

General description

A wide range of cellular processes are modulated through the generation and attachment of polyubiquitin (polyUb) chains to target proteins. Increasing evidence suggests that polyUb chains joined through linear peptide bonds between the C-terminus of a ubiquitin and the N-terminus of another play important functional roles. The enzyme machinery responsible for the generation of linear polyUb chains has been termed LUBAC, consisting of HOIL-1L and HOIP. Chains of these type have been determined to have an open conformation, similar to polyUb K63, but with very distinct functional properties. Linear polyUb chains are cleaved by the deubiquitylases CYLD, USP5 (IsoT), USP2 and have been shown to bind to many UBDs including NEMO and Trabin-n (3xnzf). Recombinant linear chains of defined length are expressed in E. coli and purified to homogeneity. Amide linkages join the N- and C-terminus of each ubiquitin molecule to each other. This molecule is HIS-tagged at the N-terminus of the most distal ubiquitin.

Physical form

In 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.

Preparation Note

Centrifuge the vial prior to opening.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
4F26L64260

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Questions

  1. Could you please provide the protein concentration of Product No. SRP6145, linear tetra-ubiquitin human, lot 7C01L64260?

    1 answer

    1. The protein concentration of Product No. SRP6145, linear tetra-ubiquitin human, lot 7C01L64260, is 1 mg/mL.

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