SRP3277

SDF-1 BETA human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): CXCL12, PBSF
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 20.0-80.0 ng/mL
• mol wt: 8.5 kDa
• packaging: pkg of 10 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: P48061
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CXCL12(6387)

Description

General description

SDF-1α and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor.

Biochem/physiol Actions

SDF-1α and β are stromal derived CXC chemokines, and signal through the CXCR4 receptor. Recombinant human SDF-1α is an 8.0 kDa protein containing 68 amino acid residues. Recombinant human SDF-1β is an 8.5 kDa protein containing 72 amino acid residues.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable