SML2234

1S,3R-RSL 3

≥98% (HPLC), powder, GPx4 inhibitor

• Synonym(s): RAS-selective lethal, RSL3, (1S, 3R)-2-(2-Chloroacetyl)-2,3,4,9-tetrahydro-1-[4-(methoxycarbonyl)phenyl]-1H-pyrido[3,4-b]indole-3-carboxylic acid methyl ester
• Empirical Formula (Hill Notation): C₂₃H₂₁ClN₂O₅
• CAS Number: 1219810-16-8
• Molecular Weight: 440.88
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD30187526
• Assay: ≥98% (HPLC)
• Form: powder

Properties

• Product Name: 1S,3R-RSL 3, ≥98% (HPLC)
• assay: ≥98% (HPLC)
• form: powder
• optical activity: [α]/D 9.0 to 15.0°, c = 0.5 in methanol
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: −20°C
• SMILES string: ClCC(=O)N1[C@H](Cc2c3c([nH]c2[C@@H]1c4ccc(cc4)C(=O)OC)cccc3)C(=O)OC
• InChI key: TXJZRSRTYPUYRW-NQIIRXRSSA-N

Description

General description

Ras-selective lethal small molecule 3 (RSL3) induces ferroptosis by inhibiting the activity of glutathione peroxidase 4 (GPx4). RSL3 not only activates ferroptosis but also blocks the activation of the mammalian target of rapamycin (mTOR) signaling pathway through interactions with GPX4. In thyroid cancer cell lines, RSL3 decreases cell viability, inhibits spheroid formation, and disrupts cell migration in vitro.

Application

1S,3R-RSL 3 has been used:

• as a glutathione peroxidase 4 (GPX4) inhibitor to study its effects on cell death in subventricular glioblastoma multiforme (GBM) lines
• GPX4 inhibitor and ferroptosis inducer to study its effects on cardiomyocyte death and heart failure in mice
• as a ferroptosis inducer in HT-1080 cellsin cell viability assay

Biochem/physiol Actions

Ferroptosis inducer; inhibits glutathione peroxidase 4 (GPX4)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable