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SAB4200100

Sigma-Aldrich

Anti-ATG13 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

ATG13 Antibody - Anti-ATG13 antibody produced in rabbit, Atg13 Antibody, Anti-Autophagy-related protein 13

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, rat, mouse

concentration

~1.0 mg/mL

technique(s)

western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KIAA0652(9776)

General description

Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2).

Specificity

Anti-Atg13 recognizes human, mouse, and rat Atg13.

Application

Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Biochem/physiol Actions

Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Physical and neurobehavioral determinants of reproductive onset and success
Day FR, et al.
Nature Genetics, 48(6), 617-617 (2016)
Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains
Nishimura T, et al.
The Embo Journal, 36(12), 1719-1735 (2017)
Di Ge et al.
Autophagy, 10(6), 957-971 (2014-06-01)
MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. No chemical activators of MTOR have been found, and the urgent challenge
ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery
Jung CW, et al.
Molecular Biology of the Cell, 20(7), 1992-2003 (2009)
Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform
Zachari M, et al.
Developmental Cell (2019)
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