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S5393

Sigma-Aldrich

Serum minus IgA/IgM/IgG human

lyophilized powder

Synonym(s):

Depleted Serum

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

human

Quality Level

conjugate

unconjugated

form

lyophilized powder

contains

15 mM sodium azide

packaging

vial of 0.5 mL

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Serum is the liquid component of blood that includes proteins, immunoglobulin and electrolytes. The product can be used for selective removal of contaminating antibodies when immobilized on a solid matrix. This product can also be used in anti-protective antigen (anti-PA) IgG measurements using PA coupled microspheres. Human serum deficient of IgG, IgA, and IgM can react specifically with anti-human whole serum but does not show reactivity with anti-human IgG, IgA, or IgM.

Application

Serum minus IgA/IgM/IgG human has been used as a control serum in immunoelectrophoresis, immunodiffusion,enzyme linked immunosorbent assay. It has also been used as
  • control sera to assess neuraminidase inhibition
  • microneutralization assay
  • single radial hemolysis (SRH) repeatability assay

Quantity

Each vial contains at least 25 mg protein

Reconstitution

Reconstitute with 0.5 mL deionized water.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Validation of Single Radial Haemolysis assay: A reliable method to measure antibodies against influenza viruses
Trombetta CM, et al.
Journal of Immunological Methods, 422, 95-101 (2015)
Age and influenza-specific pre-vaccination antibodies strongly affect influenza vaccine responses in the Icelandic population whereas disease and medication have small effects
Olafsdottir TA, et al.
Frontiers in Immunology, 8, 1872-1872 (2018)
Joanna Waldock et al.
mSphere, 6(4), e0056721-e0056721 (2021-07-29)
The hemagglutination inhibition (HAI) assay is an established technique for assessing influenza immunity, through measurement of antihemagglutinin antibodies. Improved reproducibility of this assay is required to provide meaningful data across different testing laboratories. This study assessed the impact of harmonizing
Use of lentiviral pseudotypes as an alternative to reassortant or Triton X-100-treated wild-type Influenza viruses in the neuraminidase inhibition enzyme-linked lectin assay
Biuso F, et al.
Influenza and Other Respiratory Viruses, 13(5), 504-516 (2019)
Alessandro Manenti et al.
Journal of medical virology, 92(10), 2096-2104 (2020-05-10)
The micro-neutralization assay is a fundamental test in virology, immunology, vaccine assessment, and epidemiology studies. Since the SARS-CoV-2 outbreak at the end of December 2019 in China, it has become extremely important to have well-established and validated diagnostic and serological

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