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S400HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
65546-95-4
MDL number:
MFCD00165824
UNSPSC Code:
23151817
NACRES:
NA.56

product name

Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

10-2000 kDa MW range (dextrans)
20-8000 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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General description

S400HR-750ML′s updated product is GE17-0609-01
  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F - closed cup

Flash Point(C)

38 - 43 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael J Lodes et al.
Methods in molecular medicine, 94, 91-106 (2004-02-13)
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here
An amphipathic a-helix is the principle membrane-embedded region of CTP:phosphocholine cytidylyltransferase. Identification of the 3-(trifluoromethyl)-3-(m-[<SUP>125</SUP>I]iodophenyl)diazirine photolabeled domain.
Johnson, J.E., et al.
Biochimica et Biophysica Acta - Biomembranes, 1324(2), 273-284 (1997)
Rapid Detection of Adenovirus from Fecal Specimens.
Tabish, T., et al. et al.
Methods in Molecular Medicine, 12, 1-6 (1998)
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