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RAB0810

Sigma-Aldrich

Mouse Il18 / Interleukin-18 ELISA Kit

for serum, plasma and cell culture supernatants

Synonym(s):

IFN-gamma-inducing factor, IL-18, Interleukin-18 

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.15 ng/mL
standard curve range: 0.205-50 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il18(16173)

General description

Interleukin-18 (IL-18) belongs to the IL-1 family of cytokines. It is synthesized as an inactive precursor and converted to the active form by caspase-1. IL-18 is involved in the synthesis of interferon-γ (IFN-γ) and IL-13 from T-cells and natural killer (NK) cells. It is a proinflammatory cytokine that aids in the activation of NK cells and T helper 1 cell response. The antibody pair provided in this kit recognizes mouse interleukin 18.
The antibody pair provided in this kit recognizes Mouse Interleukin 18.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse Il18 / Interleukin-18 ELISA Kit has been used to detect interleukin-18 in serum samples.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Charles A Dinarello et al.
Frontiers in immunology, 4, 289-289 (2013-10-12)
Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines. Similar to IL-1β, IL-18 is synthesized as an inactive precursor requiring processing by caspase-1 into an active cytokine but unlike IL-1β, the IL-18 precursor is constitutively present in nearly
T Yoshimoto et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(24), 13962-13966 (1999-11-26)
IL-18 is a proinflammatory cytokine that plays an important role in natural killer cell activation and T helper 1 (Th1) cell responses. Mast cells and basophils are major inducers and effectors of allergic inflammation. Here we show that basophils and
Zufeng Ding et al.
Biochemical and biophysical research communications, 451(4), 637-643 (2014-08-19)
Lectin-like oxidized low-density lipoprotein scavenger receptor-1 (LOX-1) is known to be involved in many pathophysiological events, such as inflammation. To clarify the role of LOX-1 in mtDNA damage and NLRP3 inflammasome activation, we studied wild-type (WT) and LOX-1 knockout (KO)
Benjamin Uzan et al.
EMBO molecular medicine, 6(6), 821-834 (2014-04-30)
Development of novel therapies is critical for T-cell acute leukaemia (T-ALL). Here, we investigated the effect of inhibiting the MAPK/MEK/ERK pathway on T-ALL cell growth. Unexpectedly, MEK inhibitors (MEKi) enhanced growth of 70% of human T-ALL cell samples cultured on
Jin-Mei Chen et al.
Cytokine, 119, 113-118 (2019-03-25)
Recent studies have provided overwhelming evidence of the involvement of microglia-related molecular networks in the pathogenesis of Alzheimer's diseases (AD). The potential involvement of pro-inflammatory cytokines interleukin (IL)-18, IL-23 and IL-17 on amyloid (Aβ) clearance is still unclear. In this
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