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RAB0713

Human MSLN / Mesothelin ELISA Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... MSLN(10232)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.123-30 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MSLN(10232)

General description

The MSLN ELISA kit provides for the Quantitative measurement of Mesothelin in Cell Culture Supernatants, Plasma and Serum.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

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Human MSLN / Mesothelin ELISA Kit

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Human MSLN / Mesothelin ELISA Kit

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Anti-Mesothelin antibody, Rabbit monoclonal recombinant, expressed in proprietary host, clone SP74, affinity isolated antibody

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Anti-Mesothelin antibody, Rabbit monoclonal

Anti-MSLN antibody produced in rabbit Prestige Antibodies&#174; Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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HPA017172

Anti-MSLN antibody produced in rabbit

description

-

description

liquid, clone 1A10, Calbiochem®

description

recombinant, expressed in proprietary host, clone SP74, affinity isolated antibody

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Gene Information

human ... MSLN(10232)

Gene Information

human ... MSLN(10232)

Gene Information

human ... MSLN(10232)

Gene Information

human ... MSLN(10232)

species reactivity

human

species reactivity

human

species reactivity

human (tested)

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.123-30 ng/mL

assay range

-

assay range

-

assay range

-

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

-

input

-

input

-

technique(s)

ELISA: suitable

technique(s)

-

technique(s)

immunohistochemistry: 1:100, flow cytometry: 1:100

technique(s)

immunohistochemistry: 1:200-1:500, immunoblotting: 0.04-0.4 μg/mL

detection method

colorimetric

detection method

-

detection method

-

detection method

-

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • RABTMB3
    ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS
  • RABSTOP3
    ELISA Stop Solution (Item I)
    SDS
  • RABWASH4
    20X Wash Buffer (Item B)
    SDS

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

1–2 of 2 Questions  

  1. Which amino acid residues of the MSLN protein does the antibody bind to? Does the ELISA kit recognize the mature form of MLSN protein only (~40kDa) or does it also bind to MPF?

    1 answer

    1. The epitope mapping has not been performed for the antibodies used in this kit. The antibody and standard information is included below:


      Capture Antibody Source:
      Monoclonal Mouse IgG1 Immunogen: Mouse myeloma cell line NS0-derived recombinant human Mesothelin
      Accession# AAH09272

      Detection Antibody Source:
      Polyclonal Goat IgG Immunogen: Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 1 (aa296-580)
      Accession# ABW03459

      Standard Recombinant Protein Source:
      Mouse myeloma cell line, NS0-derived human Mesothelin protein aa296-580 Accession# AAH09272

      No additional information is available regarding the variant detected by the antibodies.

      Helpful?

  2. Could you provide the molecular weight of the standard protein used in the Human MSLN ELISA (RAB0713-1KT) kit?

    1 answer

    1. The predicted molecular weight for the Mesothelin-1 standard is 33 kDa.

      Helpful?

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