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Human C5a / Complement C5a ELISA Kit

Name: Human C5a / Complement C5a ELISA Kit
Brand: SIGMA

for serum, plasma, and cell culture supernatants

Synonym(s):

Complement C5a

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About This Item

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... C5(727)

Input:

sample type plasma
sample type cell culture supernatant(s)
sample type serum

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

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Properties

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 31 pg/mL
standard curve range: 31.25-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... C5(727)

Description

General description

Human complement 5a (C5a) is generated during complement activation from the α-chain of complement C5. It binds to a signaling G-protein coupled receptor (GPCR), inducing neutrophil chemotaxis and endothelial cell activation. C5a also triggers an oxidative burst in macrophages and neutrophils and induces release of histamine in basophils and mast cells.

The antibody pair provided in this kit recognizes human Complement Component C5a.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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This Item	RAB0790	RAB0764	204902
Sigma-Aldrich RAB0631 Human C5a / Complement C5a ELISA Kit	Sigma-Aldrich RAB0790 Mouse H60a ELISA Kit	Sigma-Aldrich RAB0764 Mouse C5a / Complement C5a ELISA Kit	Sigma-Aldrich 204902 Complement C5a des-Arg, Human
description for serum, plasma, and cell culture supernatants	description -	description for serum, plasma and cell culture supernatants	description Complement C5a des-Arg, Human, is a native, single chain glycopeptide (73 aa). Once produced, C5a is converted to C5a des-Arg upon removal of the C-terminal arginine by serum carboxypeptidase N.
Gene Information human ... C5(727)	Gene Information mouse ... H60a(15101)	Gene Information mouse ... C5(15139)	Gene Information -
species reactivity human	species reactivity mouse	species reactivity mouse	species reactivity -
input sample type plasma sample type cell culture supernatant(s) sample type serum	input sample type cell culture supernatant(s) sample type serum sample type plasma	input sample type cell culture supernatant(s) sample type serum sample type plasma	input -
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 31 pg/mL standard curve range: 31.25-2000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 82 pg/mL standard curve range: 0.082-20 ng/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1.5 pg/mL standard curve range: 1.64-400 pg/mL	assay range -
detection method colorimetric	detection method colorimetric	detection method colorimetric	detection method -
technique(s) ELISA: suitable	technique(s) ELISA: suitable	technique(s) ELISA: suitable	technique(s) -