Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/
PKH67GL
PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonym(s):
Green PKH membrane labeling kit
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€1,670.00
€1,670.00
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1 KIT
€1,670.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.32
€1,670.00
Please contact Customer Service for Availability
Recommended Products
Quality Level
packaging
pkg of 1 kit
storage condition
protect from light
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Application
PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:
- To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.[1]
- To label apoptotic cells,[2] in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.[3]
- In fluorescence imaging.[4]
This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Linkage
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
Kit Components Only
Product No.
Description
- Diluent C 6 x 10
- PKH67 Cell Linker in ethanol .5 mL
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
Flash Point(F)
57.2 °F - closed cup
Flash Point(C)
14 °C - closed cup
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Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
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Articles
PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.
Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
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I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?
1 answer-
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What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?
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Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdfAlternate methods for excess dye removal have not been investigated.
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Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.
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The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.
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What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?
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PKH2 was one of the early PKH dyes. The PKH67 dye has a longer aliphatic tail. There is reduced cell-celldye transfer for PKH67 as compared with PKH2.
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What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?
1 answer-
A protocol for visualization of tissue sections can be found in the technical bulletin. For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells. If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.
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Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?
1 answer-
Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery. Methods for improving cell viability can be found on the troubleshooting guide.
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Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?
1 answer-
As long as the cell has an intact membrane, the PKH76 dye can label the cell.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?
1 answer-
In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.
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How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?
1 answer-
This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).
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