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P5147

Sigma-Aldrich

Protease from Streptomyces griseus

Type XIV, ≥3.5 units/mg solid, powder

Synonym(s):

Actinase E, Pronase E

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100 MG
€46.50
1 G
€211.00
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€772.00

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100 MG
€46.50
1 G
€211.00
5 G
€772.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

€46.50


In StockDetails


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biological source

Streptomyces griseus

Quality Level

type

Type XIV

form

powder

specific activity

≥3.5 units/mg solid

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble 0.2 mg/mL at 37 °C, clear, colorless to tan

shipped in

wet ice

storage temp.

−20°C

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Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Application

Protease from Streptomyces griseus has been used:
  • for the digestion of nucleus pulposus (NP) tissue[1]
  • in the catalysis of asymmetric one pot Mannich reaction[2]
  • for deproteinization of starch[3]
  • digestion of brain slices for the cell dissociation[4]

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Streptomyces griseus has been used in crystallographic and kinetic investigations of the covalent complex formed by tetrapeptide aldehydes and serine proteases[5].
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
The enzyme from Sigma has been used for the digestion and analysis of antithrombin-heparin complexes.[6] It has also been used for the isolation of enzyme-resistant starch.[7]
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease from Streptomyces griseus is a mixture of at least three proteolytic activities including an extracellular serine protease. Serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
This product is a mixture of at least three caseinolytic activities and one aminopeptidase activity.[8] The caseinolytic enzymes were named as Streptomyces griseus Protease A, Streptomyces griseus Protease B and Streptomyces griseus Trypsin. This product may be used when extensive or complete degradation of protein is required. This protease mixture is highly nonspecific and can digest casein to the extent of >70% as mono-amino acids.

Features and Benefits

  • highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8
  • compatible with many DNA and RNA isolation buffers
  • broad substrate specificity

Quality

Contains calcium acetate.

Physical properties

Completely inactivated by heating above 80 °C for 15-20 minutes.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Preparation Note

Collected from culture broth of S. griseus.

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Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells
Purmessur D, et al.
Arthritis Research & Therapy, 13(3), R81-R81 (2011)
Changes of AMPA receptor properties in the neocortex and hippocampus following pilocarpine-induced status epilepticus in rats
Malkin SL, et al.
Neuroscience, 327, 146-155 (2016)
Enzyme-resistant starch. I. Quantitative and qualitative influence of incubation time and temperature of autoclaved starch on resistant starch formation
Eerlingen RC, et al.
Cereal Chem., 70(3), 339-344 (1993)
Simone Valentin Hansen et al.
PloS one, 13(10), e0205461-e0205461 (2018-10-12)
Drosophila DNA replication-related element binding factor (DREF) is a transcription regulatory factor that binds the promoters of many genes involved in replication and cell proliferation and is required for normal cell cycle progression. Human DREF/zinc finger BED domain-containing protein 1
G D Brayer et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(1), 96-100 (1979-01-01)
X-ray crystallographic data show that a specific tetrapeptide aldehyde inhibitor (N-acetylprolylalanylprolylphenylalaninal) forms a stable, covalent, tetrahedral addition complex with the serine protease, SGPA, from Streptomyces griseus. Earlier proposals, based on kinetic measurements, for the covalent nature of such linkages are

Questions

1–6 of 6 Questions  
  1. Is it possible to store P5147 at -80°C after reconstitution?

    1 answer
    1. There is no specific information available about storing the enzyme at -80°C once it has been reconstituted.

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  2. Under what conditions can Product P5147, Protease from Streptomyces griseus, be used to digest protein?

    1 answer
    1. For DNA isolation, Pronase E is usually prepared as a stock solution, and prior to storage at -20°C, the solution is first heated to 56°C for about 15 minutes, then incubated at 37°C for 1 hour. This encourages self-digestion, to eliminate DNAse and RNAse contamination. The enzyme is added to a DNA sample (in the presence of 0.5-1%(w/v) SDS to disrupt DNA protein interactions) typically at 250-500 μg protein/mL, then incubated at 37°C for 1-4 hours. For protein hydrolysis, dissolve about 0.2 μmol of protein in 0.2 mL of 50 mM ammonium bicarbonate buffer at pH 8 (or phosphate buffer pH 7). Add Pronase to 1% (w/w) and incubate at 37°C for 24 hours. It may be necessary to add aminopeptidase M at 4% (w/w) and incubate at 37°C for another 18 hours.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Does Product P5147, Protease from Streptomyces griseus, contain calcium acetate?

    1 answer
    1. This product contains calcium acetate buffer salt. The amount of calcium acetate present will vary for each lot number and has been observed to range from 2 - 30% (w/w).

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  5. Is Product P5147, Protease from Streptomyces griseus, also described as "Pronase"?

    1 answer
    1. "Pronase E" is the name given to a group of proteolytic enzymes produced by Streptomyces griseus K-1. At least 10 proteases are in the mixture, five serine-type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases, and one zinc carboxypeptidase.

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  6. How should Product P5147, Protease from Streptomyces griseus, be reconstituted and how should a stock solution be prepared?

    1 answer
    1. This product can be dissolved in 0.01 M sodium acetate with 0.005 M calcium acetate at pH 7.5 at 37°C; at 0.2 mg/mL, this yields a clear solution which ranges from colorless to light tan. Calcium ion is recommended for protection from autolysis. The activity of a dilute enzyme solution containing 0.01 to 0.1 M calcium ion was stable over 24 hours at neutral pH at 2-8°C.  Pronase E is stable at 4°C for at least six months.  Stock solutions of 5 to 20 mg/mL in water are usually stored at -20°C.

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