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O5003

Sigma-Aldrich

OPI Media Supplement

Hybri-Max™, γ-irradiated, powder, suitable for hybridoma

Synonym(s):

OPI Media for cell culture

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€86.20

€86.20

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1 VIAL
€86.20

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

€86.20

Please contact Customer Service for Availability
Request a Bulk Order

grade

Hybri-Max™

Quality Level

200

sterility

γ-irradiated

form

powder

concentration

18-40 mg/vial

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

−20°C

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technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

grade

Hybri-Max™

grade

-

grade

-

grade

-

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

Application

OPI Media Supplement has been used to grow hybridoma cell lines from XenoMouse strains[1][2] and BALB/c female mice.[3]

Other Notes

Formulated with: 0.15 g oxaloacetate, 0.05 g pyruvate, and 0.0082 g insulin.

Reconstitution

Reconstitute contents of vial with 10 mL sterile water. Do not use cell culture medium for initial reconstitution. Each vial sufficient to prepare 1 liter of medium. Final working concentration: 1 mM oxaloacetate, 0.45 mM pyruvate, 0.2 U/ml insulin.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314,H317

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000311380
0000306995
0000317085
0000269101
0000260992

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Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Jun Yang et al.
Clinical biochemistry, 48(13-14), 843-848 (2015-05-31)
Neurogranin (NRGN) is a small neuronal protein that plays an important role in synaptic signaling by regulating calmodulin (CaM) availability. In this study, we developed an ELISA to measure NRGN quantitatively in serum samples from a cohort of acute traumatic
Gabriela A Canziani et al.
Analytical biochemistry, 325(2), 301-307 (2004-01-31)
Experimental and data analysis protocols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface plasmon resonance biosensor platforms. The screening methods involved capturing antibodies from crude supernatants using Fc-specific antibody surfaces and monitoring antigen binding at a
Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Tzu-Ling Sung et al.
PLoS pathogens, 5(1), e1000263-e1000263 (2009-01-17)
Cyclin T1 is a regulatory subunit of a general RNA polymerase II elongation factor known as P-TEFb. Cyclin T1 is also required for Tat transactivation of HIV-1 LTR-directed gene expression. Translation of Cyclin T1 mRNA has been shown to be
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