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MAK025

Sigma-Aldrich

Iron Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder

storage temp.

−20°C

General description

Iron is a mineral that plays an essential role in many biological processes, including DNA synthesis, electron transport and oxygen transport.[1] Iron is a transition element that can form a range of oxidation states, the most common being iron II (Fe2+ or ferrous iron) and iron III (Fe3+ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.

Application

Iron assay kit has been used to determine the concentration of Fe2+ in wild type (WT) and Δ ferric uptake regulator (Fur) strains of A. baumannii.[2]

Suitability

Suitable for the measurement of iron concentration in various samples

Principle

In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe2+ or reduced to measure total iron (Fe2+ and Fe3+). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.

Preparation Note

Kit is compatible with serum and tissue samples but not plasma samples.

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8H01K03900
8A26K03900
8C21K03900
8K12K03900
7H27K03900

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Human Ferritin ELISA Kit

Manman Xu et al.
Journal of cellular physiology, 234(4), 4232-4242 (2018-08-23)
Previous studies have demonstrated an effect of estrogen on iron metabolism in peripheral tissues. The role of estrogen on brain iron metabolism is currently unknown. In this study, we investigated the effect and mechanism of estrogen on iron transport proteins.
Ems T, Huecker MR
Biochemistry, Iron Absorption (2018)
Contributions of ferric uptake regulator Fur to the sensitivity and oxidative response of Acinetobacter baumannii to antibiotics
Ajiboye T o, et al.
Microbial Pathogenesis, 119, 35-41 (2018)
Tippapha Pisithkul et al.
mBio, 10(3) (2019-05-23)
Biofilms are structured communities of tightly associated cells that constitute the predominant state of bacterial growth in natural and human-made environments. Although the core genetic circuitry that controls biofilm formation in model bacteria such as Bacillus subtilis has been well
Bin Lan et al.
Journal of integrative medicine, 18(4), 344-350 (2020-02-29)
Our previous research showed that Naotaifang (a compound traditional Chinese herbal medicine) extract (NTE) has clinically beneficial effects on neurological improvement of patients with acute cerebral ischemia. In this study, we investigated whether NTE protected acute brain injury in rats
View All Related Papers

Questions

1–7 of 7 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is the MAK025 Iron Assay Kit able to detect iron when it is present as ferric ammonium citrate in Mycobacterium tuberculosis 7H9 broth?

    1 answer

    1. In the assay, ferric carrier protein will release ferric ions into the solution when introduced to an acidic buffer. Once these ions are reduced to the ferrous form (Fe2+), they react with Ferene S, forming a stable colored complex detectable at an absorbance of 593 nm. Consequently, the kit should theoretically be capable of detecting iron in the form of ferric ammonium citrate. It is designed to measure iron within a linear range of 0.4 to 10 nmol, or 8 µM to 200 µM iron concentration in various samples.
      It should be noted that the concentration of ferric ammonium citrate in Middlebrook 7H9 broth is generally 0.04 g/L, equivalent to 150 µmol/liter.

      Helpful?

  4. Can this assay quantify iron contained within heme directly, or is it necessary to first release the iron from the heme for detection?

    1 answer

    1. Iron attached to carrier proteins like transferrin can be quantified with the kit because these proteins will release ferric ions into the solution within an acidic buffer. However, proteins that contain iron, such as those in heme, do not dissociate in this way. As a result, it is not anticipated that iron bound within heme can be measured using this particular assay kit.

      Helpful?

  5. How can the detection of iron in mouse brain homogenate be improved when using the assay kit, given that current measurements are near or below the detection limit?

    1 answer

    1. It is suggested to reduce the quantity of homogenization buffer. For effective homogenization and cell lysis, employing a dounce homogenizer or applying mild sonication is recommended. Furthermore, using a greater quantity of tissue with a reduced amount of buffer might prove to be beneficial.

      Helpful?

  6. To homogenize mouse cells to evaluate iron content with "Iron assay kit", which type of homogenization method is best suited? Thanks

    1 answer

    1. It is recommended to use a Dounce homogenizer, not a "French press" or sonicator, or detergents or beads, for homogenization.

      Helpful?

  7. What is the range of iron concentration that can be measured?

    1 answer

    1. The detection range of this kit is 8 µM - 200 µM.

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