Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
H9658
Monoclonal Anti-HA antibody produced in mouse
clone HA-7, ascites fluid
Synonym(s):
Monoclonal Anti-HA, HA Antibody - Monoclonal Anti-HA antibody produced in mouse, Ha Antibody Sigma, Sigma Ha Antibody, Anti-HA, Anti-Influenza Hemagglutinin
Sign Into View Organizational & Contract Pricing
Select a Size
100 μL
€484.00
0.2 ML
€624.00
All Photos(5)
Select a Size
Change View
100 μL
€484.00
0.2 ML
€624.00
About This Item
conjugate:
unconjugated
application:
ELISA (i)
ICC
IP
WB
ICC
IP
WB
clone:
HA-7, monoclonal
technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
citations:
895
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HA-7, monoclonal
contains
15 mM sodium azide
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Looking for similar products? Visit Product Comparison Guide
General description
The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.
Specificity
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:
- immunoprecipitation in human embryonic kidney 293 cells
- immunoblotting in HeLa cells
- immunopurification in human embryonic kidney 293 cells
- immunostaining for electron microscopy in mouse embryonic fibroblasts
- in immunocytochemistry of hippocampal slices
Monoclonal Anti-HA antibody produced in mouse has been used in:
- Immunoblotting, to study the expression of fusion protein
- Coimmunoprecipitation assay
- Immunoblotting of lysate protein
Biochem/physiol Actions
Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.
Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Calmodulin shuttling mediates cytonuclear signaling to trigger experience-dependent transcription and memory
Cohen SM, et al.
Nature Communications, 9(1), 2451-2451 (2018)
Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls.
Fouchier R A M, et al.
Journal of Virology, 79(5), 2814-2822 (2005)
A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity.
Glaser L, et al.
Journal of Virology, 79(17), 11533-11536 (2005)
Synaptic targeting and functional modulation of GluK1 kainate receptors by the auxiliary neuropilin and tolloid-like (NETO) proteins.
Copits B A, et al.
The Journal of Neuroscience, 31(20), 7334-7340 (2011)
Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency Situations.
Metwally A and Yousif A
International Journal of Molecular Sciences, 18(4), 766-766 (2017)
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
1 answer-
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
Helpful?
-
-
What is the recommended dilution of Product H9658 - HA antibody for immunoprecipitation and for indirect immunostaining?
1 answer-
H9658 - Monoclonal Anti-HA antibody produced in mouse is the ascites fluid. It is not purified. We only test this product in western blotting. This product contains 15-55 mg/ml total protein with 4-7 mg/ml specific antibody. H3663 - Monoclonal Anti-HA antibody produced in mouse as the purified immunoglobulin. We test this product for immunoprecipitation using 0.5-2.0 μg and for immunocytochemistry using 1.0-2.0 μg/mL.These ranges would be a good starting concentration. In order to obtain best results in different techniques and preparations, we recommend determining optimal working dilution by titration test.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
