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H1138

Sigma-Aldrich

Horse Serum

Donor Herd, USA origin, Heat inactivated, sterile-filtered, suitable for cell culture

Synonym(s):

HS, equine sera, equine serum, horse sera, sera, serum

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100 ML
€60.70
500 ML
€184.00
6 X 500 ML
€1,030.00

€60.70


Please contact Customer Service for Availability


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100 ML
€60.70
500 ML
€184.00
6 X 500 ML
€1,030.00

About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71
biological source:
horse serum
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice

€60.70


Please contact Customer Service for Availability

biological source

horse serum

Quality Level

sterility

sterile-filtered

composition

Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL Endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Horse Serum has been used:
  • as a media supplement in the differentiation medium to culture the pheochromocytoma (PC12) cells
  • in immunocytochemistry and immunohistichemistry
  • to study the therapeutic effect of serum treatment on cardiomyocyte viability

Preparation Note

Collected from a controlled herd.
Prepared by heating at 56 °C for 30 minutes.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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N Saito et al.
The Journal of infection, 46(1), 49-55 (2002-12-31)
Helicobacter pylori (H. pylori), present in a half of the world's population, is a very successful pathogen. The infection by this bacterium causes several gastric diseases in human. H. pylori is morphologically divided into two types; a spiral and a
Joerg Jores et al.
The Journal of infectious diseases, 219(10), 1559-1563 (2018-12-13)
Capsular polysaccharides have been confirmed to be an important virulence trait in many gram-positive and gram-negative bacteria. Similarly, they are proposed to be virulence traits in minimal Mycoplasma that cause disease in humans and animals. In the current study, goats
Nathan J Marchant et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(22), 7447-7457 (2014-05-30)
In human alcoholics, abstinence is often self-imposed, despite alcohol availability, because of the negative consequences of excessive use. During abstinence, relapse is often triggered by exposure to contexts associated with alcohol use. We recently developed a rat model that captures
Alberto Perez-Alvarez et al.
Nature communications, 11(1), 2464-2464 (2020-05-20)
Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge.
Julien Dufort-Gervais et al.
Scientific reports, 10(1), 6956-6956 (2020-04-26)
Synapse loss occurs early and correlates with cognitive decline in Alzheimer's disease (AD). Synaptotoxicity is driven, at least in part, by amyloid-beta oligomers (Aβo), but the exact synaptic components targeted by Aβo remain to be identified. We here tested the

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Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Why is serum Heat-Inactivated (HI)?

    1 answer
    1. Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

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