G9880

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

• Synonym(s): ALT1, L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
• CAS Number: 9000-86-6
• Enzyme Commission number: 2.6.1.2 (BRENDA, IUBMB)
• Beilstein: 3562140
• EC Number: 232-561-4
• MDL number: MFCD00131192
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Porcine heart
• form: ammonium sulfate suspension
• specific activity: ≥75 units/mg protein
• mol wt: 42 kDa
• foreign activity: L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%; glutamic-oxalacetic transaminase ≤0.05%
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.

Biochem/physiol Actions

GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄ solution, pH 6.0

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves