G8764

Gc-globulin from human plasma

≥90% (SDS-PAGE), lyophilized powder (containing sodium chloride and sodium phosphate buffer salt)

• Synonym(s): DBP, Group-Specific Component, Vitamin D Binding Protein
• CAS Number: 68476-36-8
• MDL number: MFCD00131143
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: human plasma
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: lyophilized powder (containing sodium chloride and sodium phosphate buffer salt)
• mol wt: ~52 kDa
• technique(s): LC/MS: suitable
• solubility: H₂O: soluble 2 mg/mL
• UniProt accession no.: P02774
• storage temp.: −20°C
• Gene Information: human ... GC(2638)

Description

General description

Gc-Globulin, a member of the albumin superfamily, is the major plasma carrier of vitamin D and its metabolites. It is also, along with gelsolin, part of the extracellular actin scavenger system. Gc-globulin also binds monomeric actin (G-actin) with high affinity to scavenge actin released by necrotic cells, preventing its polymerization into filaments. Gc-globulin enhances the chemoattractant activity of C5a for inflammatory cells.

Gc-globulin from human plasma is encoded by the gene GC, which is mapped to human chromosome 4q13.3.[1]

Gc-globulin or the vitamin D binding protein (DBP) is a 51-58 kDa multifunctional serum glycoprotein synthesized in large quantities by primarily in the liver.[2] It is a member of the albumin gene family and has the characteristic multiple disulfide-bonded, triple domain structure.[3]

Application

Gc-globulin from human plasma has been used:

• as a fluorescent probe for G-actin staining[4]
• as a fluorescent probe to monitor steady-state rate of actin filament disassembly[5]
• as a calibrator for liquid chromatography with mass spectrometry (LC-MS/MS)[6]

Gc-globulin is a multifunctional, albumin-like plasma protein that often requires cell surface binding to mediate some of its diverse functions.[7] It has been used to anlayze its glycosylation patterns.

Biochem/physiol Actions

Gc-Globulin, a member of the albumin superfamily, is the major plasma carrier of vitamin D and its metabolites. It is also, along with gelsolin, a part of the extracellular actin scavenger system. Gc-globulin also binds monomeric actin (G-actin) with high affinity to scavenge actin released by necrotic cells, preventing its polymerization into filaments. Gc-globulin enhances the chemoattractant activity of C5a for inflammatory cells.

Single nucleotide polymorphism in the gene GC is associated with deficiency of 25-hydroxyvitamin D [25(OH)D].[1]

Preparation Note

Native Gc-globulin from human plasma

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable