FG1385

RPMI-1640 Medium

HEPES modification, with stable glutamine, sterile liquid, suitable for cell culture

• Synonym(s): Roswell Park Memorial Institute 1640 medium
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• product name: RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES and stable glutamine, liquid, sterile-filtered
• Quality Level: 400
• sterility: sterile-filtered; sterile
• form: liquid
• technique(s): cell culture | mammalian: suitable
• components: stable glutamine: yes; phenol red: yes; HEPES: 25 mM; NaHCO₃: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable