F4512

Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Rabbit Anti-Human IgG (whole molecule)−Fluorescein isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162487
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF
• Citations: 12

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunofluorescence: 1:64-1:128 using Hep2 cells
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. FITC (fluorescein isothiocyanate), a fluorochrome dye conjugation is useful in localization of antigen-antibody interaction of biological structures like cells or tissues. Anti-Human IgG (whole molecule) −FITC antibody diluted 1:100 in wash buffer is used in flow cytometry to analyze antibody affinity and virus binding. This antibody can also be used in indirect immunofluorescence antibody test. FITC labeled anti-human IgG antibody reacts specifically with human.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Human IgG (whole molecule)−FITC antibody is specific for human IgG. Rabbit Anti-Human IgG is conjugated to crystalline fluorescein isothiocyanate (FITC) in an alkaline reaction and then further purified to remove free FITC.

Immunogen

IgG from pooled normal human serum

Application

Anti-Human IgG (whole molecule) −FITC antibody may be used as secondary antibody in indirect immunofluorescence and is also used in flow cytometry .

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

The antibody may be used for immunofluorescent staining of human peripheral blood lymphocytes at a working dilution of 1:64. A minimum working dilution of 1:64 was used on acetone fixed mouse liver cells and anti-nuclear antibody positive serum as the primary antibody. For flow cytometry to label NIH3T3 cells, the antibody was used at a working dilution of 1:100. The antibody was also used in ELISA using human sera.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)