F4049

ANTI-FLAG^® M2-FITC antibody, Mouse monoclonal

clone M2, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: M2, monoclonal
• Application: FLAG purification, IF (d)
• Citations: 114

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous solution
• species reactivity: all
• concentration: ~1 mg/mL
• technique(s): FLAG purification: suitable, direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.

Application

Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.

Learn more product details in ourFLAG^® application portal.

Physical form

Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable