HF2x653

90012609, Human x Mouse Hybridoma hybrid, Lymphoblast

• UNSPSC Code: 41106514

Properties

• product name: HF2x653, 90012609
• biological source: Human x Mouse Hybridoma hybrid
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human (WI-L2-729-FF2) x mouse (P3X63/Ag8.653) heterohybridoma

Cell Line Description

Generated by electro-acoustic fusion of 2 lines. Parent: WI-L2-729-HF2. Murine myeloma: P3X63/Ag8.653. Non-immunoglobulin secreting, HAT-sensitive, resistant to ouabain at greater than 100uM, will fuse with normal human lymphocytes and lymphoblastoids.

Application

Fusion partner

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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