The Ehrlich-Lettre Ascites strain E cell line is banked with the UK Health Security Agency and maintained as part of the European Collection of Authenticated Cell Cultures. Applications such as intraperitoneal injection are not tested. For application information, consult the relevant literature.
Ehrlich-Lettre Ascites strain E cell line
87032503, mouse unknown/unspecified, Epithelial
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About This Item
UNSPSC Code:
41106514
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Product Name
Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 87032503
biological source
mouse unknown/unspecified
packaging
tube of 5 μg 87032503-DNA-5UG
pkg of vial of cells 87032503-1VL
growth mode
Adherent
karyotype
2n = 40
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
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Cell Line Origin
Mouse carcinoma
Cell Line Description
Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.
Application
Anti-tumour test and tumourigenicity studies
Culture Medium
NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Media change 3 times a week.
Other Notes
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Is this "E" cell line suitable for an in vivo mouse Ehrlich ascites tumor model? What should we do before i.p. injection? Can the entire thawed cell sample be injected i.p. into a mouse, or are additional steps required before in vivo proliferation?
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