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product name
WEHI-231, 85022107
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Surface Immunoglobulin M (IgM)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse B cell lymphoma
Cell Line Description
Expresses surface IgM but does not secrete Ig. Secretion of IgM can be induced with LPS. WEHI 231 is a B cell lymphoma of BALB/c x NXB F1 origin induced by mineral oil injection.
Application
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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