C9983

Creatine Kinase BB Fraction human

≥90% (SDS-PAGE), liquid, recombinant, expressed in Pichia pastoris

• MDL number: MFCD02677919
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• recombinant: expressed in Pichia pastoris
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• mol wt: 35-50 kDa
• concentration: ≥1.0 mg/mL
• UniProt accession no.: P12277
• storage temp.: −70°C
• Gene Information: human ... CKB(1152)

Description

General description

Solution in 50% glycerol, Tris buffer with 10 mM Bis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA

Application

Human creatine kinase BB fraction has been used to investigate the survival benefit of the late percutaneous coronary intervention in patients after acute myocardial infarction. Human creatine kinase BB fraction has also been used in a study to analyze protein oxidations and resultant loss of function.

Biochem/physiol Actions

The product is used as a control in monitoring levels in cerebrospinal fluid by ELISA. Creatine kinase (CK) BB is a member of the CK gene family and is a cytosolic CK isoform present in the brain. The molecular mass is found to be 35-50 kDa. CKs catalyze the reversible transfer of a phosphoryl group between ATP and creatine. It plays an important role in the regulation of ATP level in neural cells. Therefore, a decrease in CK BB activity is one of the biochemical markers of the CNS cell damage in age-related neurodegenerative diseases such as Alzheimer′s disease, Pick′s disease, and Lewy body dementia.

Unit Definition

One unit will transfer 1.0 μmole of phosphate from creatine phosphate to ADP per minute at 37 °C (measured at 340 nm as one equimolar amount of NADH produced by coupled reaction).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable