Yes, as stated following the product name, these cells are isolated from a single donor.
C-12921
Human CD34+ Progenitor Cells (hCD34+-CB)
Isolated from cord blood, single donor, 100,000 cryopreserved cells
Synonym(s):
hCD34+-CB cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Recommended Products
biological source
human umbilical cord blood
packaging
pkg of 100,000 cells
morphology
(progenitor)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
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Cell Line Origin
Cord Blood
Application
CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. PromoCell offers CD34+ Progenitor Cells from the cord blood of healthy donors. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.
Quality
Rigid quality control tests are performed for each lot of CD34+ Progenitor Cells. They are routinely characterized by flow cytometry analyzing the viability, cell size, and the presence of significant markers, i.e. CD34. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
Click here for more information.
Other Notes
Recommended Plating Density: 5000 cells per mlTested Markers: CD34 positive
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Certificates of Analysis (COA)
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Tatjana Weidner et al.
Nature protocols, 16(7), 3210-3240 (2021-04-14)
Receptor targeting of vector particles is a key technology to enable cell type-specific in vivo gene delivery. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them
Articles
CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.
Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.
Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.
Protocols
In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
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Does it come from a single donor?
1 answer-
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I would like to know whether these are CD34+ progenitor cells isolated from cord blood and directly shipped after isolation or whether they are shipped after being cultured.
1 answer-
Yes, these cells are CD34+ progenitor cells isolated from cord blood. PromoCell Blood and Blood Progenitor Cells are cryopreserved directly after isolation (=P0). Customers will receive them cryopreserved. They haven’t been in culture before freezing.
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