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C0794

Sigma-Aldrich

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

Synonym(s):

Chitosan N-acetylglucosaminohydrolase

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10 UNITS
€629.00

About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

€629.00


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form

buffered aqueous glycerol solution

specific activity

≥15 units/mg protein (E1%)

concentration

≥0.5 mg/mL

solubility

0.1 M acetate buffer, pH 5.5: soluble

storage temp.

−20°C

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.[1]

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Unit Definition

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5[1]

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Boucher, I., et al.
The Journal of Biological Chemistry, 270, 31-31 (1995)
Tahira Naz et al.
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Canthaxanthin is a reddish-orange xanthophyll with strong antioxidant activity and higher bioavailability than carotenes, primarily used in food, cosmetics, aquaculture, and pharmaceutical industries. The spiking market for natural canthaxanthin promoted researchers toward genetic engineering of heterologous hosts for canthaxanthin production.
Aretz, W., et al.
FEMS Microbiology Letters, 65, 31-31 (1989)
Marie-Pierre Dubeau et al.
Journal of bacteriology, 193(10), 2441-2450 (2011-03-29)
A palindromic sequence is present in the intergenic region preceding the chitosanase gene csnA (SSPG_06922) of Streptomyces lividans TK24. This sequence was also found in front of putative chitosanase genes in several other actinomycete genomes and upstream genes encoding putative
Shou-Kuo Hsu et al.
Journal of agricultural and food chemistry, 60(2), 649-657 (2011-12-15)
Two thermally stable chitosanase isoforms were purified from the sheaths of chitosan-treated bamboo shoots. Isoforms A and B had molecular masses of 24.5 and 16.4 kDa and isoelectric points of 4.30 and 9.22, respectively. Using chitosan as the substrate, both

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