B6048

Restriction Endonuclease Buffer SP

Name: Restriction Endonuclease Buffer SP
Brand: SIGMA

2 ×

Synonym(s):

Restriction Endonuclease Buffer

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About This Item

UNSPSC Code:

12352204

Key Documents

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InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI key

YNZJHLFHFNCZIE-UHFFFAOYSA-N

SMILES string

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

grade

Molecular Biology

description

2× buffer contains 200 mM Tris-HCl, pH 7.6, 20 mM MgCl₂, 300 mM NaCl, 2 mM dithiothreitol at 37 °C.

form

buffered aqueous solution

concentration

2 ×

storage temp.

−20°C

Quality Level

100

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1 of 4

This Item	B8781	S2015	T9650
Sigma-Aldrich B6048 Restriction Endonuclease Buffer SP	Sigma-Aldrich B8781 Restriction Enzyme Buffer SB	Sigma-Aldrich S2015 SSPE Buffer 20× Concentrate	Sigma-Aldrich T9650 Tris Acetate-EDTA buffer
grade for molecular biology	grade for molecular biology	grade for molecular biology	grade for molecular biology
concentration 2 ×	concentration 10 ×	concentration -	concentration -
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 200
form buffered aqueous solution	form buffered aqueous solution	form solution	form solution
storage temp. −20°C	storage temp. −20°C	storage temp. room temp	storage temp. -
description 2× buffer contains 200 mM Tris-HCl, pH 7.6, 20 mM MgCl₂, 300 mM NaCl, 2 mM dithiothreitol at 37 °C.	description 10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl₂, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.	description -	description -

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Lot/Batch Number

099H1153

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