A1543

Anti-Human IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Goat Anti-Human IgG (whole molecule)−AP
• MDL number: MFCD00162453
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• recombinant: expressed in goat
• conjugate: alkaline phosphatase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:20,000; dot blot: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot (chemiluminescent): 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains, with molecular weight of 50kDa and 25kDa respectively. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG binds to human IgG. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains.

Immunogen

IgG purified from human serum

Application

Goat anti-human IgG can also be used for dot blot (1:30,000) and immunohistochemical (1:50) applications.

Serum human IgG levels were analyzed by solid phase ELISA assays using alkaline phosphatase-conjugated goat anti-human IgG antibody incubated for 1 hour at 37°C. Assays was developed using 0.1% p-nitrophenyl phosphate (Sigma) as the substrate. Antibodies to the Seoul (strain 80/39) virus was detected in human sera by western blot analysis using alkaline phosphatase-conjugated goat anti-human IgG antibody at a 1:1000 dilution. Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indoyl-phosphate substrate (Sigma).

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable