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Key Documents

94740

Sigma-Aldrich

Vanadyl ribonucleoside complexes

BioReagent, for molecular biology

Synonym(s):

Ribonucleoside vanadyl complexes, VRC

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250 MG
€119.00
1 G
€380.00

€119.00

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250 MG
€119.00
1 G
€380.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

€119.00

Please contact Customer Service for Availability

grade

for molecular biology

Quality Level

100

product line

BioReagent

form

powder

suitability

in accordance for ribonuclease inhibition test

shipped in

wet ice

storage temp.

−20°C

General description

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.[2][3][4]

Biochem/physiol Actions

The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity[1] These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.[5]

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD5801
BCBC5675V
BCBC5675
1140341
47698/1

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Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
Zhang X, et al.
Oncology Letters, 8(4), 1693-1696 (2014)
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant DNA technology
Puskas R S, et al.
Biochemistry, 21(19), 4602-4608 (1982)
Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization
Hu F, et al.
Oncology Letters, 9(6), 2795-2798 (2015)
Tissue array for Tp53, C-myc, CCND1 gene over-expression in different tumors
Liu G Y, et al.
World Journal of Gastroenterology, 14(47), 7199-7199 (2008)
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Questions

1–2 of 2 Questions  

  1. Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?

    Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?

    1 answer

    1. This product is a complex that has an estimated molecular weight of 422.5 g/mole. The solubility at 400 mM has not been tested. This product is offered in a prepared solution under product 94742 at a concentration of 200 mM. Based on the estimated molecular weight, a 0.4 M (400 mM) solution would have a concentration of 169 mg/mL. Solutions may be prepared in nuclease-free PBS containing 15% glycerol or 5% DMSO as a cryoprotectant. Solutions may then be stored in working aliquots at −20˚C. Avoid the use of EDTA in all buffer solutions as this will cause degradation of the complex. See the link below to publication that may be helpful. http://diyhpl.us/~bryan/irc/protocol-online/protocol-cache/view_cache.cgi%3FID=3486

      Helpful?

  2. How to make 200 mM from 94740-250MG vanadyl ribonucleoside complexes powder?

    1 answer

    1. The method to prepare a 200mM solution of the 94740 is proprietary and involves in-process testing. However, product 94742 and R3380 are prepared 200mM solutions of Vanadyl Ribonucleoside complexes.

      Helpful?

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