94581
Hirudin recombinant from yeast
lyophilized powder, white, >1500 U/vial
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![[Tyr(SO3H)63]-Hirudin Fragment 54-65 ≥95% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/401/056/a0ac1972-7f9e-45b9-8e32-74f3f275e097/640/a0ac1972-7f9e-45b9-8e32-74f3f275e097.png)
biological source
synthetic
yeast (Pichia pastoris)
form
lyophilized powder
specific activity
>1500 U/vial
mol wt
Mr ~6963.5
color
white
solubility
0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless
storage temp.
−20°C
Application
Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay.
Biochem/physiol Actions
Recombinant hirudin appears to be antigenic. Independent studies have found that 50-70% of patients treated for several days with recombinant hirudin develop at least one hirudin antibody isotype. The antibodies reduce the anticoagulant effect of hirudin, but prolong its plasma half-life by reducing renal filtration.
The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.
Unit Definition
1 U corresponds to 1 ATU (antithrombin unit; 1 ATU is defined as the amount of inhibitor that neutralizes 1 NIH unit of thrombin).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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