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93290

TRIS borate – EDTA buffer solution

for Northern and Southern blotting, solution

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Synonym(s):

TBE buffer

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Size/SKUAvailabilityPrice
1 L
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€88.60
5 L
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€410.00

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25
eCl@ss:
32129211

€88.60

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grade

Molecular Biology

Quality Segment

description

long run, concentrate

product line

BioReagent

form

solution

quality

10× concentrate, ready-to-use solution

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.6-9.0

storage temp.

room temp

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Preparation Note

The TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

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This Item
51309T4415T9285
TRIS borate – EDTA buffer solution for Northern and Southern blotting, solution

Sigma-Aldrich

93290

TRIS borate – EDTA buffer solution

TRIS borate-EDTA buffer concentrate, long run for Northern and Southern blotting, solution

Sigma-Aldrich

51309

TRIS borate-EDTA buffer concentrate, long run

Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

Sigma-Aldrich

T4415

Tris-Borate-EDTA buffer

Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting

Sigma-Aldrich

T9285

Tris-EDTA buffer solution

description

for Northern and Southern blotting, solution

description

for Northern and Southern blotting, solution

description

BioReagent, suitable for electrophoresis, 10× concentrate

description

100X concentrate for Northern and Southern blotting

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

Molecular Biology

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

form

solution

form

solution

form

solution

form

liquid

pH

8.6-9.0

pH

-

pH

-

pH

7.8-8.2 (1 × in water)

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

room temp

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H360FD

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

10043-35-3

CAS No.

10043-35-3

CAS No.

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    Certificates of Analysis (COA)

    Lot/Batch Number
    017120/1
    048368/1
    014839/1
    012884/1
    BCCK0303

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