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77618
Phenol – chloroform – isoamyl alcohol mixture
BioUltra, Molecular Biology, 49.5:49.5:1
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
phenol:Chloroform:Isoamyl Alcohol
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Please contact Customer Service for Availability | €116.00 |
500 mL | Please contact Customer Service for Availability | €418.00 |
About This Item
CAS Number:
NACRES:
NA.07
PubChem Substance ID:
UNSPSC Code:
12352401
MDL number:
Grade:
Molecular Biology
grade
Molecular Biology
Quality Segment
product line
BioUltra
form
liquid
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
pH
7.7-8.3(H2O-phase, after extraction with H2O, 1:1)
density
1.29 g/mL at 20 °C
absorption
cut-off at 360 nm
storage temp.
2-8°C
SMILES string
ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
InChI
1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
Application
- Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).
- Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).
- The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).
- The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).
Physical form
mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline
1 of 1
This Item | |||
|---|---|---|---|
| description BioUltra, Molecular Biology, 49.5:49.5:1 | description BioUltra, Molecular Biology, 125:24:1 | description BioUltra, Molecular Biology, 25:24:1 | description BioUltra, Molecular Biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC) |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| form liquid | form liquid | form liquid | form liquid |
| density 1.29 g/mL at 20 °C | density 1.12 g/mL at 20 °C | density 1.28 g/mL at 20 °C | density - |
| pH 7.7-8.3(H2O-phase, after extraction with H2O, 1:1) | pH 4-5(H2O-phase, after extraction with H2O, 1:1) | pH 7.2-8.3(H2O-phase, after extraction with H2O, 1:1) | pH - |
| impurities phosphatases, none detected, DNases, none detected, RNases, none detected, proteases, none detected | impurities proteases, none detected, DNases, none detected, phosphatases, none detected, RNases, none detected | impurities phosphatases, none detected, DNases, none detected, RNases, none detected, proteases, none detected | impurities insoluble matter, passes filter test, DNases, none detected, phosphatases, none detected, ≤0.001% free alkali (as NH3), RNases, none detected, proteases, none detected |
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Signal Word
Danger
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
Target Organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point (°F)
177.8 °F - closed cup
Flash Point (°C)
81 °C - closed cup
PPE (personal protective equipment)
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
67-66-3
CAS No.
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