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77398

Sigma-Aldrich

(R)-Oxynitrilase, Linum usitatissimum, recombinant from Pichia pastoris

solution (clear), slightly yellow, ≥50 U/mL

Synonym(s):

(R)-Acetone cyanohydrin Lyase

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About This Item

MDL number:
UNSPSC Code:
12352204

recombinant

expressed in Pichia pastoris

form

solution (clear)

concentration

≥50 U/mL

color

slightly yellow

storage temp.

2-8°C

Biochem/physiol Actions

R-oxynitrilase catalyzes the enantioselective addition of HCN to aldehydes or ketones.

Components

contains ~20 mM sodium acetate, ~20 mM ZnCl2, ~1 M NaCl, pH ~5.7

Unit Definition

1 U corresponds to the amount of enzyme which cleaves 1 μmol acetone cyanhydrin to cyanide and acetone per minute at pH 4.2 and 30 °C

Other Notes

Investigation of the substrate range

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Albrecht et al.
Biotechnology and applied biochemistry, 17 ( Pt 2), 191-203 (1993-04-01)
The purification of (R)-oxynitrilase (EC 4.1.2.10) from Linum usitatissimum (flax) has been improved considerably. The enzyme is obtained from seedlings in 60% yield by fractional salt precipitation followed by ion-exchange and hydrophobic-interaction chromatography. Final gel-permeation chromatography yields a protein with

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