39294

Fluo 3-AM solution

1 mM in DMSO, ≥99.0% (TLC)

• Empirical Formula (Hill Notation): C₅₁H₅₀Cl₂N₂O₂₃
• CAS Number: 121714-22-5
• Molecular Weight: 1129.85
• MDL number: MFCD00083336
• UNSPSC Code: 12171500
• NACRES: NA.32

Properties

• Quality Segment: 100
• assay: ≥99.0% (TLC)
• form: liquid
• concentration: 1 mM in DMSO
• fluorescence: λ_ex 506 nm; λ_em 525.0 nm±5.0 nm in 0.1 M Tris pH 8.0, esterase
• storage temp.: −20°C
• SMILES string: Clc1[c](cc2[o]c3c(c(c2c1)c4cc(c(cc4)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOc5c(ccc(c5)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)cc(c(c3)OCOC(=O)C)Cl)=O
• InChI: 1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2
• InChI key: PTPUOMXKXCCSEN-UHFFFAOYSA-N

Description

General description

Cell permeable fluorescent indicator of intracellular Ca²⁺; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca²⁺; lower binding affinity allows measurement of higher peaks of Ca²⁺ transients than with Fura 2.

Application

suitable as fluorescent Ca sensitive probe (after cleavage of the AM ester)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: 188.6 °F
• flash_point_c: 87 °C