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901767

Sigma-Aldrich

PC biotin-PEG3-NHS ester

Synonym(s):

PC biotin-NHS ester

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About This Item

Empirical Formula (Hill Notation):
C36H52N6O15S
CAS Number:
Molecular Weight:
840.89
UNSPSC Code:
51171641
NACRES:
NA.22

form

solid

reaction suitability

reaction type: click chemistry
reagent type: cross-linking reagent

mp

170 °C

storage temp.

−20°C

Application

PC biotin-NHS ester is a unique amine-reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

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Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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