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11814320001

Roche

Formamide

=99%, liquid, pH 4-10 (20 °C, 200 g/L), suitable for hybridization

Synonym(s):

Formamide, Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
75-12-7
Molecular Weight:
45.04
Beilstein:
505995
MDL number:
MFCD00007941
UNSPSC Code:
41116100
PubChem Substance ID:
329749563

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

Assay

99%

form

liquid

autoignition temp.

932 °F

mol wt

45.04

packaging

pkg of 500 mL

manufacturer/tradename

Roche

technique(s)

hybridization: suitable

color

colorless

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

absorption

≤0.08 at 290 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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General description

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.[1]

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Formamide has been used in:
in situ hybridization[2][3]
  • as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis[4]
  • for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay[5]
  • as a component of stop buffer used in cleavage and polyadenylation activity assays[6]

Quality

Contaminants: ≤5 ppm heavy metals (as Pb), ≤5 ppm Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H351,H360D,H373

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
94020520
94015620
940140
74014420
20J2056766

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Fluorescence in situ hybridization (FISH) protocol in human sperm
Sarrate Z and Anton E
Journal of Visualized Experiments, (31), e1405-e1405 (2009)
Zaida Sarrate et al.
Journal of visualized experiments : JoVE, (31)(31), doi:10-doi:10 (2009-09-03)
Aneuploidies are the most frequent chromosomal abnormalities in humans. Most of these abnormalities result from meiotic errors during the gametogenic process in the parents. In human males, these errors can lead to the production of spermatozoa with numerical chromosome abnormalities
Nucleic acid reassociation in formamide.
B L McConaughy et al.
Biochemistry, 8(8), 3289-3295 (1969-08-01)
mRNA deadenylation is coupled to translation rates by the differential activities of Ccr4-Not nucleases
Webster MW, et al.
Molecular Cell, 70(6), 1089-1100 (2018)
Weiwei Yang et al.
eLife, 10 (2021-11-09)
Shotgun metagenomic sequencing is a powerful approach to study microbiomes in an unbiased manner and of increasing relevance for identifying novel enzymatic functions. However, the potential of metagenomics to relate from microbiome composition to function has thus far been underutilized.
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