The SCT125 is neither a pre-stain nor a loading dye. According to the data sheet, the recommendation is to first run the gel, and then immerse it in a 1 to 10,000 dilution of SCT125 post-electrophoresis. Instructions for its use can be found on the product's data sheet, found in the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/mm/sct125#product-documentation
SCT125
GelGreen® Nucleic Acid Stain 10000X Water
GelGreen is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Synonym(s):
Fluorescent DNA gel stain
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0.5 ML
€149.00
About This Item
UNSPSC Code:
12352106
NACRES:
NA.52
Recommended Products
form
liquid
technique(s)
PCR: suitable
solubility
water: soluble
suitability
suitable for nucleic acid staining
detection method
fluorometric
General description
GelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 1). Thus, GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen is far more sensitive than SYBR Safe (Figure 2). Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally.
The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen is not classified as hazardous waste.
Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader
Emission: Long path green filter such as a SYBR filter or GelStar filter.
The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen is not classified as hazardous waste.
Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader
Emission: Long path green filter such as a SYBR filter or GelStar filter.
Application
GelGreen is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Nucleic acid detection
Research Category
Cell Culture
Cell Culture
Research Sub Category
Live Cell Dye
Live Cell Dye
Quality
Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader
Emission: Long path green filter such as a SYBR filter or GelStar filter.
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader
Emission: Long path green filter such as a SYBR filter or GelStar filter.
Physical form
Liquid
Storage and Stability
GelGreen is a very stable dye. Store GelGreen at room temperature, protected from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50°C for two minutes and vortex. Protect From Light.
Legal Information
GelGreen is a registered trademark of Biotium Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F - (Dimethylsulfoxide)
Flash Point(C)
87 °C - (Dimethylsulfoxide)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Customers Also Viewed
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Is SCT125 used as a pre-stain mixed into the gel prior to the electrophoresis run, or is it a loading dye that should be added to the samples immediately before being loaded into the wells?
Is SCT125 used as a pre-stain mixed into the gel prior to the electrophoresis run, or is it a loading dye that should be added to the samples immediately before being loaded into the wells?
1 answer-
Helpful?
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Would gel green work for a gel reader that uses blue light instead of UV light? The excitation needs to be about 480 nm
1 answer-
Yes, this product compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader).
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