PC253L
Anti-Calbindin D-28K (Ab-1) Rabbit pAb
lyophilized, Calbiochem®
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
baboon, monkey, rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CALB1(793)
rhesus monkey ... Calb1(677721)
General description
Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Recognizes the Calbindin D-28K protein in rat hippocampus tissue.
Immunogen
Bovine
purified bovine cerebellum calbindin D-28K protein
Application
Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Warning
Toxicity: Standard Handling (A)
Physical form
Undiluted serum.
Reconstitution
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat striatum, hippocampus, or cortex
Rat striatum, hippocampus, or cortex
Other Notes
Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Conde, F., et al. 1994. J. Comp. Neurol.341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ana I Arroyo et al.
EMBO molecular medicine, 6(3), 398-413 (2014-01-23)
Understanding the role of lipids in synapses and the aberrant molecular mechanisms causing the cognitive deficits that characterize most lipidosis is necessary to develop therapies for these diseases. Here we describe sphingomyelin (SM) as a key modulator of the dendritic
Daniela Herrera Moro Chao et al.
PloS one, 10(9), e0138107-e0138107 (2015-09-30)
Gaucher disease is characterized by lysosomal accumulation of glucosylceramide due to deficient activity of lysosomal glucocerebrosidase (GBA). In cells, glucosylceramide is also degraded outside lysosomes by the enzyme glucosylceramidase 2 (GBA2) of which inherited deficiency is associated with ataxias. The
Hongtao Zhang et al.
Developmental cell, 48(6), 780-792 (2019-03-12)
FAT4 mutations lead to several human diseases that disrupt the normal development of the kidney. However, the underlying mechanism remains elusive. In studying the duplex kidney phenotypes observed upon deletion of Fat4 in mice, we have uncovered an interaction between
Patrick W Keeley et al.
Visual neuroscience, 34, E002-E002 (2017-01-10)
Retinal bipolar cells spread their dendritic arbors to tile the retinal surface, extending them to the tips of the dendritic fields of their homotypic neighbors, minimizing dendritic overlap. Such uniform nonredundant dendritic coverage of these populations would suggest a degree
B A Patel et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 22(8), 909-918 (2010-05-21)
Inhibitory neurotransmission to the longitudinal muscle is more prominent in the neonatal than in the adult guinea pig ileum. Inhibitory neuromuscular transmission was investigated using in vitro ileal longitudinal muscle myenteric plexus (LMMP) preparations made from neonatal (< or =48
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service