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MAIPN4550

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, non-sterile

Synonym(s):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate, PVDF Membrane Plate

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50 PKG
€1,510.00

€1,510.00

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50 PKG
€1,510.00

About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

€1,510.00

Please contact Customer Service for Availability

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

400

description

Non-sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for Avidin-biotin Linkages, DNA-binding Proteins, Protein Binding & PAMPA. Comes in a pack of 50.

sterility

non-sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

parallel artificial membrane permeation assay (PAMPA): suitable

filtration area

0.3 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

shipped in

ambient

Application

MultiScreen®-IP Filter Plate, 0.45 μm, clear, non-sterile has been used:
  • as artificial membrane support and a receiver plate in parallel artificial membrane permeation assays (PAMPA) to determine the permeabilities of oximes[1]
  • in enzyme-linked immunospot (ELISpot) assay[2]
  • in keyhole limpet hemocyanin (KLH)-specific ELISPOT for the identification of antigen (Ag)-specific cells[3]

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Claudia Giesecke et al.
Journal of immunology (Baltimore, Md. : 1950), 200(12), 3981-3992 (2018-05-08)
There are currently limited insights into the progression of human primary humoral immunity despite numerous studies in experimental models. In this study, we analyzed a primary and related secondary parenteral keyhole limpet hemocyanin (KLH) immunization in five human adults. The
A C Belkina et al.
Journal of dental research, 99(7), 855-862 (2020-03-19)
Periodontitis (PD) is a common source of uncontrolled inflammation in obesity-associated type 2 diabetes (T2D). PD apparently fuels the inflammation of T2D and associates with poor glycemic control and increased T2D morbidity. New therapeutics are critically needed to counter the
Hee Chun Jeong et al.
Bioorganic & medicinal chemistry, 17(17), 6213-6217 (2009-08-12)
A series of fluorinated oxime compounds was designed and synthesized in order to probe the effect of fluorine substitution on reactivation of inhibited acetylcholinesterase (AChE) by organophosphorus agents. Permeability measurements, using the Parallel Artificial Membrane Permeation Assays (PAMPA) method, were

Articles

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

Permeability & Solubility Analysis Workflow

Combining solubility & PAMPA assays streamlines drug permeability testing.

Protocols

HDM-PAMPA with the MultiScreen Permeability Filter Plate

Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller and Kansy such assays provide rapid, low cost and automation friendly methods to measure a compound’s passive permeability.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Questions

  1. Could you please explain the meaning of the terms "donor," "acceptor," "receiver," and "collection" in relation to MAIPN4550 and MAIPN4510 filter plates?

    1 answer

    1. The donor plate is also known as the filter plate, while the acceptor plate can be referred to as the acceptor, receiver, or collection plate. For detailed information on the PAMPA assay, you can refer to the provided link. Additionally, a datasheet is available that reviews some of the donor and acceptor plates. The donor plates include MAIPN4550 and MAIPNTR10. An alternative donor plate option is S2EM004M99, which is a 'MA' classic designed plate. It is important to note that any plate starting with 'MS---' in the catalog number cannot be used.

      As for the acceptor plates, several options are available, such as MSSACCEPT0R, MATRNPS50, and MSCPNUV40.

      Moreover, tech notes for the automation of PAMPA-Lipid on the Sciclone ALH 3000 workstation and the automation of PAMPA-HDM using a MultiScreen® Filter Plate on a Beckman Biomek® FX Workstation are also available. An article titled "Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)" provides further insights into the topic.

      Helpful?

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