MABT341

Anti-CD226/DNAM-1 Antibody, clone NewE1 (Azide Free)

clone NewE1, from mouse

• Synonym(s): CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD_antigen=CD226
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: NewE1, monoclonal
• species reactivity: human
• technique(s): activity assay: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_006557
• UniProt accession no.: Q15762
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CD226(10666)

Description

General description

CD226/DNAM1 is a T cell expressed plasma membrane receptor protein that plays multiple roles in lymphocyte signaling, intercellular adhesion, lymphokine secretion and cytotoxic T-cell and NK cell mediated killing. CD226 activates natural killer (NK) cell-mediated cytotoxicity and is a key player in the immune synapse between killer T cells and targets. Also, because it recognizes CD155 and nectins CD226/DNAM1 is being examined as a potential anti-cancer agent because soluble CD226 directly inhibits tumor growth by binding CD155 expressed on tumor cells and down regulating proliferation. CD226/DNAM-1 is expressed on Th1, but not Th2 or Th0 cells, it is also expressed on NK cells, CD8+ T-cells, but not developing T cells. CD226/DNAM-1 is also expressed on monocytes and platelets.

Immunogen

Recombinant protein corresponding to human CD226/DNAM-1.

Application

Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet activation and aggregation (Sherrington, P.D., et al. (1997). J. Biol. Chem. 272:21735-21744).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD226/DNAM-1 in isolated surface proteins from Jurkat and platelets (Sherrington, P.D., et al. (1997). J. Biol. Chem. 272:21735-21744).
MABT341 works by western blotting under both non-reducing and reducing conditions.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-CD226/DNAM-1 Antibody, clone NewE1 (Azide Free) is validated for use in Western Blotting and Immunoprecipitation and Activity Assay for the detection of CD226/DNAM-1.

Quality

Evaluated by Western Blotting in CD226 Transfected CHO cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 Transfected CHO cell lysate.

Target description

~39 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable