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FC014

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
bovine
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
1.0 mg/mL
Assay:
~95% (affinity purified, SDS-PAGE)
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Product Name

Fibronectin, Bovine,

biological source

bovine

Quality Level

100

assay

~95% (affinity purified, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1.0 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)

UniProt accession no.

P02751, P07589

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This Item
F4759ECM001F2006
Fibronectin, Bovine

Sigma-Aldrich

FC014

Fibronectin, Bovine

Fibronectin bovine plasma powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F4759

Fibronectin bovine plasma

Fibronectin human recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Sigma-Aldrich

ECM001

Fibronectin human

Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F2006

Fibronectin human plasma

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

bovine

biological source

bovine plasma

biological source

human

biological source

human plasma

concentration

1.0 mg/mL

concentration

≥1 mg protein/mL

concentration

-

concentration

>1 mg/mL protein

assay

~95% (affinity purified, SDS-PAGE)

assay

-

assay

≥95% (SDS-PAGE)

assay

≥85% (SDS-PAGE)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

powder

form

lyophilized powder

form

lyophilized powder

General description

Bovine fibronectin was purified from bovine plasma over a gelatin agarose column. A single band at 220kDa (reduced) is identified, corresponding to intact fibronectin under reduced conditions.
Product Source: Bovine plasma

Application

ELISA, Ouchterlony and immunoblotting.

Physical form

Liquid in a solution of 2M Urea in 0.05 M Tris pH 7.0.

Preparation Note

Aliquot and store up to 12 months at -20°C. For short term (1-2 weeks) use, storage at 2-8°C is acceptable. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Ruoslahti et al.
The Biochemical journal, 193(1), 295-299 (1981-01-01)
Human fibronectin was isolated from second-trimester amniotic fluid, from amniotic fluid obtained at term and from adult plasma. The amniotic-fluid fibronectins had a slightly higher apparent molecular weight on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis than the plasma fibronectin. Early- and late-amniotic-fluid

Protocols

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Global Trade Item Number

SKUGTIN
FC01404053252269929

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