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Sigma-Aldrich

Goat Anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed

0.5 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

biotin conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

ELISA: suitable
immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Specificity

Reacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains and mouse immunoglobulins.
The antibody reacts with human kappa light chains as demonstrated by ELISA and flow cytometry.

Immunogen

Epitope: Ig kappa chain
Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with κ light chains.

Application

Immunofluorescence: ≤ 1 μg/106 cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies
This Goat anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA, IF for the detection of Human Ig κ chain.

Physical form

Affinity Chromatography
Purified by affinity chromatography on pooled human Igs with κ light chains covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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