TREX1, (DNase III), or three prime repair exonuclease 1 is the major 3’→ 5’ DNA exonuclease in mammalian cells. TREX1 binds preferentially to ssDNA and can also remove mismatched 3’ terminal deoxyribonucleotides at DNA strand breaks. The protein is required for proper DNA checkpoint signaling and plays critical roles in DNA replication and gap filling during DNA repair. TREX1 has a perinuclear distribution in mammalian cells and is colocalized with ER-associated proteins in cells at rest. TREX1 relocalizes to the nucleus and specifically to replication forks during S phase and periods of DNA synthesis and repair such as after irradiation. TREX1 acts on an ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates and thus acts to attenuate DNA damage checkpoint signaling and prevent pathological immune activation in normal cells. Defects in TREX1 protein have been associated in humans with many inherited diseases including Aicardi-Goutiėres syndrome type 1 (AGS1), systemic lupus erythematosus (SLE), chilblain lupus type 1 (CHBL1), and vasculopathy, retinal, with cerebral leukodystrophy (RVCL). EMD Millipore’s Anti-TREX1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human samples.
Immunogen
KLH-conjugated linear peptide corresponding to human TREX1.
Application
Detect DNASE using this rabbit polyclonal antibody, Anti-TREX1 Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected TREX1 in human spleen tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected TREX1 in human spleen tissue.
Quality
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TREX1 in 15 µg of human spleen tissue lysate.
Target description
~30 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~32 kDa, ~33 kDa, and ~39 kDa
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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