ABE572
Anti-N6-methyladenosine (m6A) Antibody
from rabbit
Synonym(s):
m6A
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity (predicted by homology)
all
technique(s)
ChIP: suitable
RIP: suitable
dot blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse.
Immunogen
BSA-conjugated N6-methyladenosine.
Application
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in RNA containing N6-methyladenosine (Dominissini, D., et al. (2012). Nature. 485(7397):201-206.; Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).
(RNA) Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in methylated RNA containing N6-methyladenosine (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).
Dot Blot Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in a panel of modified oligonucleotides (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.; Jia, G., et al. (2011). Nat Chem Biol. 7(12):885-887.).
(RNA) Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in methylated RNA containing N6-methyladenosine (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).
Dot Blot Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in a panel of modified oligonucleotides (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.; Jia, G., et al. (2011). Nat Chem Biol. 7(12):885-887.).
Detect N6-methyladenosine (m6A) using Anti-N6-methyladenosine (m6A) Antibody validated for use in Dot Blot, Chromatin IP (ChIP) & RNA Binding Protein IP (RIP).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Quality
Evaluated by Dot Blot in oligomers containing N6-methyladenosine.
Dot Blot Analysis: 1 µg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
Dot Blot Analysis: 1 µg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Andrea Orioli et al.
Genome research, 26(5), 624-635 (2016-03-05)
RNA polymerase III (Pol III) is tightly controlled in response to environmental cues, yet a genomic-scale picture of Pol III regulation and the role played by its repressor MAF1 is lacking. Here, we describe genome-wide studies in human fibroblasts that
Xiaoling Liu et al.
Analytical chemistry, 90(9), 5546-5551 (2018-04-14)
N6-methyladenine (6mA) is a rediscovered DNA modification in eukaryotic genomes. To explore the distribution and functions of 6mA, it is of paramount option to use immunoprecipitation to select 6mA-containing DNA fragments for genome-wide sequencing. Presumably, most of the 6mA-free fragments
Hua Su et al.
BMC genomics, 21(1), 39-39 (2020-01-15)
Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m6A-modified. The abundance of circRNAs was influenced by m6A. Furthermore, the significance of m6A
Jérôme Lannes et al.
Scientific reports, 6, 31563-31563 (2016-08-20)
The GnRH neurohormone is the main activator of the pituitary gonadotropins, LH and FSH. Here we investigated the contribution of microRNAs in mediating GnRH activation. We first established that miR-125b targets several actors of Gαq/11 signalling pathway, without altering Gαs
Belinda Baquero-Perez et al.
eLife, 8 (2019-10-28)
N6-methyladenosine (m6A) is the most abundant internal RNA modification of cellular mRNAs. m6A is recognised by YTH domain-containing proteins, which selectively bind to m6A-decorated RNAs regulating their turnover and translation. Using an m6A-modified hairpin present in the Kaposi's sarcoma associated
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