34321-M

FITC, Isomer I

• Synonym(s): FITC, Isomer I, Fluorescein Isothiocyanate, Isomer I
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 3326-32-7
• Molecular Weight: 389.38
• MDL number: MFCD00005063
• UNSPSC Code: 41116133

Properties

• Assay: ≥95% (TLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: orange-red
• solubility: DMSO: 5 mg/mL; DMF: soluble; aqueous buffer, pH ≥ 9.0: soluble
• storage temp.: 2-8°C
• SMILES string: S=C=Nc1cc2c(cc1)C3(OC2=O)c4c(cc(cc4)O)Oc5c3ccc(c5)O
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9). Fluorescence EMmax: 520 ± 3 nm (pH 9).

Popular derivative of fluorescein. Can be used to label antibodies and other proteins.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Cherry, N.B., et al. 1965. Stain Technol. 44, 179.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable