The recommended dilution for 20-116 Sigma-Aldrich Kinase Inhibitor Cocktail is 1:5, preferably in the cell extraction buffer, so it is already present during lysis. Here are a couple of articles where the inhibitor cocktail has been used:
- Carcinogenesis. 2007 Jul;28(7):1408-17. Epub 2007 Mar 19.
- Carcinogenesis. 2008 Mar;29(3):473-9. Epub 2007 Oct 4.
20-116
Kinase Inhibitor Cocktail
liquid, for the inhibition of serine-threonine phosphatase, ATPases, alkaline and acid phosphatases and protein-phosphotyrosine phosphatases. This small molecule/inhibitor is primarily used for Biochemicals applications.
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About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41
form:
liquid
€262.00
Please contact Customer Service for Availability
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Product Name
Kinase Inhibitor Cocktail, The Inhibitor Cocktail controls the biological activity of kinases. This small molecule/inhibitor is primarily used for Biochemicals applications.
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
shipped in
wet ice
Quality
Routinely evaluated in kinase assays as an inhibitor.
Physical form
20mM MOPS, pH 7.2, 25mM β-glycerol phosphate, 5mM EGTA, 1mM sodium orthovanadate, 1mM dithiothreitol.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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DYRK3 activation, engagement of protein kinase A/cAMP response element-binding protein, and modulation of progenitor cell survival.
Ke Li,Shuqing Zhao,Vinit Karur,Don M Wojchowski
The Journal of Biological Chemistry null
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