05-366

Anti-PLCγ-1 Antibody, clone B-6-4

clone B-6-4, 1 mg/mL, Upstate^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: B-6-4, monoclonal
• Application: IP, WB
• Citations: 28

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: B-6-4, monoclonal
• species reactivity: human, rabbit, rat, bovine, mouse
• manufacturer/tradename: Upstate^®
• concentration: 1 mg/mL
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable

Description

General description

~135 kDa

Immunogen

Purified soluble phospholipase C (PLC) g-1 from bovine brain

Application

Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Biochem/physiol Actions

PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H312 + H332,H412
• pcodes: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1